Fast Cycling
So fast and powerful..they could be the first SuperMixes to face mandatory drug testing.
PerfeCTa® FastMixes® set a new standard for high efficiency qPCR results with a significantly faster protocol for increased productivity.
About PerfeCTa® FastMixes®
PerfeCTa® qPCR FastMixes® are 2X concentrated, ready-to-use reaction cocktails that deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using either fast or conventional cycling protocols. Rapid cycling is achieved by instant activation of AccuFast™ Taq DNA polymerase coupled with rapid polymerization kinetics. The result is qPCR in as little as 33 minutes. PerfeCTa® qPCR FastMixes® are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye.
Protocol Comparison: Conventional vs. Fast
| Conventional Reagents | PerfeCTa® FastMix® | |
| Activation 95°C | 600-900s | 20s |
| PCR Cycling 95°C/60°C | 15s/60s | 1-3s/20s |
| Time for 40 cycles (excl. ramp time) | 52 min | 14 min |
| Total Run Time: iCycler iQ® | 80-103 min (4-6 runs/8 hr shift) | 40 min (12 runs/8 hr shift) |
| Total Run Time: Eppendorf Mastercycler® |
72-82min (5-6 runs/8 hr shift) | 33 min (14 runs/8 hr shift) |
Fast Cycling qPCR
Get up to 3x faster cycle times and 5x greater sensitivity.
Probe Detection| PerfeCTa® qPCR FastMix®, UNG |
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| PerfeCTa® qPCR FastMix®, UNG, ROX™ |
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| PerfeCTa® qPCR FastMix®, UNG, Low ROX™ |
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Detection using SYBR Green I
| PerfeCTa® SYBR® Green FastMix® for iQ™ |
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| PerfeCTa® SYBR® Green FastMix® |
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| PerfeCTa® SYBR® Green FastMix®, ROX™ |
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| PerfeCTa® SYBR® Green FastMix®, Low ROX™ |
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Fast Cycling One-Step qRT-PCR 
Quanta Biosciences is introducing the first One-Step Fast qRT-PCR kits.
One-Step qRT-PCR Kits
