Quanta's Extracta sample preparation system is a system that can be scaled to process various amounts of solid tissue in a simple and fast single-tube procedure. DNA may be purified from many tissue types and is suitable for use in sensitive downstream applications such as PCR, qPCR and High Resolution Melt Analysis.

Features and Benefits

Simple, fast protocol
Compatible with laboratory automation
DNA is suitable for qPCR, High Resolution Melt Analysis, endpoint PCR and other applications
Works with wide range of tissue including hair, buccal swabs and mouse tails
Optional 10 minute protocol for high-throughput applications

Extracta Technology

Extracta DNA Prep for PCR is a two-component reagent kit for rapid extraction of PCR-ready genomic DNA from mammalian tissues. Tissues are processed in 35 minutes with little hands-on time. Compared to a conventional proteinase-K process this is significantly faster with fewer handling steps. The genomic DNA is suitable for sensitive downstream PCR applications including end-point PCR, High Resolution Melt Analysis (HRM) and quantitative real-time PCR (qPCR) without requiring any additional clean-up. In addition the extracted DNA may be used in multiplexed PCR applications such as transgene or knock-out analyses. Tissue extractions can be done in tubes, plates or deep-well blocks to allow for adaptation to workflow and automation on liquid-handling workstations.