Home -> PerfeCTa™ qPCR FastMix™ & qScript 1-Step Fast qRT-PCR

Fast Cycling qPCR

Get up to 3x faster cycle times and 5x greater sensitivity with PerfeCTa™ SYBR® Green FastMix™ and PerfeCTa™ FastMix™.

Fast Cycling qRT-PCR

Quanta Biosciences is introducing the first One-Step Fast qRT-PCR kits for fast-
cycling parameters and uncompromised sensitivity and specificity.

About qScript Fast One-Step Kits

• The fastest One-Step kits in the industry
• The first kits available in MGB-probe formulations
• The only One-Step kits available enabling duplexing with VIC endogenous controls
• Unparalleled sensitivity enables use of lower reaction volumes and probe concentrations
• Ability to detect rare or dilute targets including virus and pathogens

Performance Data

qScript™ One-Step Fast qRT-PCR Kit

Rapid One-Step qRT-PCR with Broad Dynamic Range and High Sensitivity (ACTB)
Beta actin mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (1 µg to 1 pg) in 10-µL volumes with the qScript™ One-Step Fast qRT-PCR Kit and FAM-BHQ1 labeled 5’-hydrolysis probe. Triplicate reactions for each RNA quantity were incubated for 5 min at 50ºC followed by 30s at 95ºC and then 40 cycles of 3s, 95ºC; 20s, 60ºC on a Mastercycler® ep realplex S (Eppendorf). Total run time was under 41 minutes.

More Performance Data

• qScript™ One-Step Fast qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)

qScript™ One-Step Fast MGB qRT-PCR Kit

Rapid One-Step qRT-PCR with Broad Dynamic Range and High Sensitivity (GAPD)
Glyceraldehyde-3-phosphate dehydrogenase mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (1 µg to 1 pg) in 10-µL volumes with the qScript™ One-Step Fast MGB qRT-PCR Kit and GAPD (FAM-MGB) TaqMan® Endogenous Control primer/probe set (Applied Biosystems). Triplicate reactions for each RNA quantity were incubated for 5 min at 50ºC followed by 30s at 95ºC and then 40 cycles of 3s, 95ºC; 20s, 60ºC on a Mastercycler® ep realplex S (Eppendorf). Total run time was under 41 minutes.

More Performance Data

• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. TaqMan® One-Step RT-PCR Master Mix Reagents Kit (Applied Biosystems)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. QuantiFast™ Probe RT-PCR Kit (Qiagen)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)

About PerfeCTa™ FastMixes™

PerfeCTa™ FastMixes™ set a new standard for high efficiency qPCR results with a significantly faster protocol for increased productivity. PerfeCTa™ qPCR FastMixes™ are 2X concentrated, ready-to-use reaction cocktails that deliver maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using either fast or conventional cycling protocols. Rapid cycling is achieved by instant activation of AccuFast™ Taq DNA polymerase coupled with rapid polymerization kinetics. The result is qPCR in as little as 33 minutes. PerfeCTa™ qPCR FastMixes™ are available for all real-time PCR instrument platforms including those requiring normalization with ROX reference dye.

Performance Data PerfeCTa™ qPCR FastMixes™

PerfeCTa™ SYBR® Green FastMix™

Fast-cycle SYBR® Green qPCR with equal performance to conventional SYBR Green protocols
RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa™ SYBR Green FastMix™, with fast cycling protocol, or PerfeCTa™ SYBR Green SuperMix, with conventional cycling conditions.

More Performance Data

• PerfeCTa SYBR Green FastMix comparison to SYBR GreenER qPCR SuperMix.
• PerfeCTa™ SYBR® Green FastMix™ Comparison to iQ™ SYBR Green SuperMix
• PerfeCTa™ SYBR® Green FastMix™ Comparison to SYBR PreMix Ex Taq™
• Quanta Fast Cycling vs. Quanta Standard Cycling
• PerfeCTa™ SYBR® Green FastMix™ Comparison to DyNAmo™ Flash SYBR Green PCR Kit
• PerfeCTa™ SYBR® Green FastMix™ Comparison to QuantiFast™ SYBR Green PCR Kit
• Faster, More Sensitive SYBR Green qPCR

PerfeCTa™ qPCR FastMix™

Fast-cycle TaqMan® qPCR with equal performance to conventional protocols.
RPLPO was amplified from log-fold serial dilutions of qScript™-synthesized cDNA from HeLa cell total RNA (100 ng to 100 fg ) with either PerfeCTa™ qPCR FastMix (fast cycling protocol) or PerfeCTa™ qPCR SuperMix (conventional cycling conditions) and the RPLPO TaqMan Endogenous Control primer/probe set (Applied Biosystems). Average plots for triplicate reactions of each input quantity are shown.

More Performance Data

• High-Performance TaqMan Results in Less Time and Lower Reaction Volumes.