Extracta DBS

PCR-ready genomic DNA from dried blood spots

Features & Benefits

  • Optimized for DNA extraction from dried blood spot punches
  • Single reagent for PCR-ready DNA in 30 minutes
  • Maximized assay sensitivity, lower Cq values, when combined with Quantabio ToughMix®
  • Compatible with high-throughput automation for PCR, qPCR and NGS applications

Extracta DBS is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Extracta DBS is a ready-to-use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman 903 filter paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing (1-3) reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix or PerfeCTa MultiPlex qPCR ToughMix enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

Combine wtih ToughMix and maximize qPCR assay sensitivity

Extracta DBS is the perfect match with Quantabio ToughMix for sensitive and precise target quantification. The crude extraction combined with ToughMix, a Quantabio master mix that is tolerant to common PCR inhibitors, results in higher DNA yields independent of DNA sample inputs and qualities to enable accurate detection and high sensitivity even with low copy targets.

Reliable, Consistent Lot-to-Lot Performance

Low amounts and quality of DNA recovered from dried blood spots commonly restrict the utilization of DNA. To overcome these limitations, Extracta DBS increases the yield and quality allowing for efficient and reliable recovery of DNA from dried blood spots. Manufactured under stringent ISO 13485 standards, Extracta DBS ensures uniform lot-to-lot performance resulting in reliable reproducibility in combination with Quantabio ToughMix.

Performance Data

Extracta DBS Workflow


Fast, single solution extraction workflow in 30 min

Extracta DBS DNA extraction from dried blood spots workflow. PCR-ready DNA is eluated from dried blood spots with less handling in 30 minutes.     


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Extracta DBS TREC quantification: uniform lot performance


Different Extracta DBS preparation

This figure demonstrates consistent lot-to-lot performance in a TREC quantification assay using genomic DNA extracted  from dried blood spots. Lot-to-lot performance was tested for High, Medium and Low copy number TREC samples. The results highlight the reliability and reproducibility across various product lots which are attributed to Quantabio’s high manufacturing and production standards under ISO 13485.     


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Extracta DBS TREC Quantification


TREC Control Sample

Illustrates the results of a T-cell Receptor Excision Circles (TREC) Assay using Extracta DBS and PerfeCTa ToughMix. Samples were generated using dried blood spot punches following the Extracta DBS protocol and used subsequently for quantification of T-cell Receptor Excision Circles. The samples are representatives of High, Medium and Low TREC copy numbers along with the corresponding Cq values.


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Details

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    FAQs
    How should the extracted DNA be measured?
    Measurement of DNA by methods using DNA binding dyes or absorbance at 260 nm are not applicable to a crude lysis method such as the Extracta DBS method. The recommended way to accurately quantify DNA in an Extracta DBS lysate is to measure a specific DNA sequence (generally a single-copy gene in gDNA) by qPCR.
    What is the stability of the extracted DNA?
    We have no data on the stability of the extracted DNA. We recommend that the DNA be used for downstream applications as soon as possible following extraction.
    Can the extracted DNA be stored?
    We recommend using the DNA in downstream applications immediately after extraction. If this is not possible, the extracted DNA can be refrigerated at 2-8°C for short term storage (1-2 days) or stored frozen at -15 to -25°C for prolonged storage. We have no data on the stability of extracted DNA after prolonged storage.
    For what downstream applications can the extracted DNA be used?
    The extracted DNA is compatible with a variety of end-point PCR, real-time PCR, next generation sequencing, or Sanger sequencing procedures.
    What Quantabio products have confirmed compatibility with DNA extracted by the Extracta DBS kit?
    The following Quantabio products have been demonstrated to perform well with the extracted DNA: Accustart Genotyping ToughMix (95115, 95116, 95117) PerfeCTa Multiplex qPCR ToughMix (95147, 95148, 95149) PerfeCTa qPCR ToughMix (95112, 95113, 95114, 95138, 95139, 95140)
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