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qScript™ cDNA Synthesis Kits set a new standard for reproducibility, specificity, speed and sensitivity in qPCR and conventional RT-PCR. Key to the performance of qScript™ cDNA Synthesis Kits is our proprietary qScript™ Reverse Transcriptase, a mixture of an MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. Go directly to the corresponding cDNA Synthesis product list.

• Unrivaled sensitivity - unbiased representation of all RNA targets regardless of abundance
• Rapid protocols - qScript™ methods are faster and deliver the most reproducible results
• Flexible priming options - choose from kits pre-optimized with oligo dT/random primer mixtures or optimize your own priming method with our qScript™ Flex cDNA Synthesis Kit

The first "true" SuperMix format commercially available for cDNA synthesis. qScript™ cDNA SuperMix offers superior sensitivity, accuracy, and quantitative features. For increased convenience, throughput and reproducibility. Convenience - single tube reaction and streamlined protocol.

• Available in ready-to-use 96 well plates or single tube format. Simply add RNA and incubate
• Ideal for high-throughput applications
• Broad dynamic range - optimized SuperMix allows accurate conversion of RNA to cDNA regardless of RNA input
• Optimized for qPCR using total RNA or polyA selected RNA as template
• Ideal for dilute and low-copy samples - 5X reaction mix maximizes RNA input volume

qScript™ cDNA SuperMix: Performance Data

Linearity - ACTB
qScript cDNA Supermix was used for first-strand cDNA synthesis using log-fold serial dilutions of HeLa cell total RNA from 1 µg to 1 pg. Eight replicate cDNA reactions were performed for each input quantity of RNA. 1/10th of each first-strand reaction was used for qPCR of the ACTB gene using PerfeCTa™ SYBR Green SuperMix.

More Performance Data

• Reproducibility - CDKN1B
• Sensitivity - TRRAP comparison to SuperScript® III SuperMix First Strand SuperMix for qPCR & QuantiTect Reverse Transcription Kit
• Higher Yield (more accurate representation) of low abundance gene with qScript - PP2A
• Protocol Comparison
• Simple and Reproducible High Through Put cDNA Synthesis

qScript™ cDNA Synthesis Kit: Performance Data

Broad Linear Dynamic Range
qScript cDNA Synthesis Kit was used for first-strand cDNA synthesis using log-fold serial dilutions of HeLa cell total RNA from 1 µg to 1 pg. Six replicate cDNA reactions were performed for each input quantity of RNA. 1/10th of each first-strand reaction was used for qPCR of the b-actin gene using PerfeCTa™ SYBR Green SuperMix.

More Performance Data

• Consistent cDNA Synthesis Efficiency - cDNA serial dilution vs. total RNA serial dilution
• High Capacity cDNA Synthesis.

qScript™ Flex cDNA Kit: Performance Data

Long RT-PCR - Reproducible long cDNA Synthesis with oligo dT primer and qScript™ Flex cDNA Synthesis Kit.
First-strand synthesis was carried out as 12 separate reactions using oligo dT primer and 1 µg HeLa total RNA template according to the qScript Flex cDNA Synthesis Kit instructions. Immediately following first-strand synthesis, 2 µl (1/10th) of each reaction was used as template for amplification of either an 8.2 kb or 6 kb fragment of the APC gene with a long PCR SuperMix. 50 µl-PCRs were cycled for 1 min at 94°C followed by 30 cycles of 94°C, 20s; 58°C, 20S, 68°C, 10 min. 10 µl of each PCR was analyzed by 0.8% agarose, 0.5X TBE gel electrophoresis and ethidium bromide staining.

More Performance Data

• Exceptional cDNA length, simple protocol
• Comparison to SuperScript® III SuperMix First Strand SuperMix (Invitrogen) b-actin
• Linearity