UltraPlex 1-Step ToughMix

Tough-tested, multiplex, 1-step real time PCR

Features & Benefits

  • 4X concentrated master mix allows greater flexibility with dilute samples - as little as 1 pg of total RNA.
  • Multiplexing capable with up to 5 targets
  • Tough-tested for applications where PCR inhibition from template impurities may be a concern, such as: crude extracts, clinical specimens, or environmental samples.
  • Temperature stabilized master mix enables convenient reaction setup at ambient room temperature.


Ultraplex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.


  • Contents

    UltraPlex 1-Step ToughMix Low ROX (4X), containing:

    • 4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
    • Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
    • Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).
  • Storage & Handling
    UltraPlex 1-Step ToughMix Low ROX is stable for up to one year when stored in a constant temperature freezer at ‑20ºC protected from light. Please refer to the CoA or box label for the expiration date. Repeated freezing and thawing does not affect RT-qPCR performance.
  • Instrument Capability
    • Applied Biosystems 5700
    • Applied Biosystems 7000
    • Applied Biosystems 7300
    • Applied Biosystems 7700
    • Applied Biosystems 7900
    • Applied Biosystems 7900HT
    • Applied Biosystems 7900 HT Fast
    • Applied Biosystems StepOne™
    • Applied Biosystems StepOnePlus™
    Low ROX
    • Applied Biosystems 7500
    • Applied Biosystems 7500 Fast
    • Stratagene Mx3000P®
    • Stratagene Mx3005P™
    • Stratagene Mx4000™
    • Applied Biosystems ViiA 7
    • Applied Biosystems QuantStudio™
    • Agilent AriaMx
    • Douglas Scientific IntelliQube®
    • QIAGEN Rotor-Gene Q
    No ROX
    • BioRad CFX
    • Roche LightCycler 480
    • Other
    Bio-Rad iCycler iQ systems
    • BioRad iCycler iQ™
    • BioRad MyiQ™
    • BioRad iQ™5
  • Related Resources