qScript XLT 1-Step RT-qPCR ToughMix

Tough-tested 1-step real time PCR

Features & Benefits

  • Tough Tested - Overcomes common inhibitors including polysacharides, heme/hemoglobin, humic acid, melanin
  • Flexible - Use fast or standard qPCR cycling conditions
  • Broad dynamic range- Reliable data from your precious samples every time
  • Multiplexing enabled - Supports highly sensitive detection for up to four targets


qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters. This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies. Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
Featured Publication

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Open full publication including video

What Customers Say
"We used the qScript XLT 1-step RT-qPCR ToughMix to reliably and very quickly identify an experimental drug candidate and demonstrate its efficacy to correct the debilitating effect of an inherited disease. The immediate success and impeccable performance of this mix shortened our labs time to result and confirmation easily by 2-3 months."
Lead Scientist / Researcher
TransLab, Children’s Hospital Boston


Ordering Information