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AccuStart II PCR ToughMix

Robust, reliable PCR assay performance with challenging sample materials or impure templates
Features & Benefits
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification
  • Separate electrophoretic mobility dye reduces risk of post-PCR cross contamination with gel electrophoresis

 

AccuStart II PCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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AccuStart II PCR ToughMix

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Kit Size:  100 x 25 µL rxns (1 x 1.25 mL)
Part Number:  95142-100
Price:  $105.00
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Kit Size:  800 x 25 μL rxns (8 x 1.25 mL)
Part Number:  95142-800
Price:  $841.00
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Kit Size:  4000 x 25 μL rxns (1 x 50 mL)
Part Number:  95142-04K
Price:  $2,529.00
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Description

AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. The only user supplied components are DNA template and molecular grade water.

A key component to AccuStart PCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. These antibodies bind the polymerase and keep it inactive prior to the initial PCR denaturation step. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Similar to Taq DNA polymerase, the activated polymerase in AccuStart II PCR ToughMix possesses 5'>3' DNA polymerase activity and a double-strand specific 5'>3' exonuclease. The polymerase does not have 3'-exonuclease activity and is free of any contaminating endo or exonuclease activities. PCR products generally contain non-templated dA additions and can be cloned using vectors that have a single 3'-overhanging thymine residue on each end.

 

GelTrack Loading Dye is a mixture of blue and yellow electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp. This optional component simplifies post-PCR analysis with gel electrophoresis and eliminates potential for cross contamination by enabling direct transfer of PCR products to the gel sample wells.

Details

Details

Contents
  • 2X concentrated PCR SuperMix containing reaction buffer with optimized concentrations of molecular-grade MgCl2, dNTPs, AccuStart II hot start Taq DNA polymerase, ToughMix additives and stabilizers.
  • GelTrack Loading Dye: 50X concentrated mixture blue and yellow electrophoresis-tracking dyes. (Does not contain a density reagent.)
Customer Testimonials

Customer Testimonials

AccuStart II PCR ToughMix

"Good product, worked well on difficult to amplify template."

Research Associate | University of Texas at Austin
AccuStart II PCR ToughMix

"This is an extremely good enzyme mix while testing side by side with Invitrogen's similar enzyme mix. The extension time is ridiculously good with as much as 5 sec for 0.5 kb."

Ph.D. Student | Goethe-Universität Frankfurt am Main
AccuStart II PCR ToughMix

"We had a great result with AccuStart II PCR ToughMix running HRM PCR with tomato crude DNA extract."

Research Scholar | North Carolina State University
AccuStart II PCR ToughMix

"My experiments demonstrated that AccuStart II PCR Toughmix improved the yield of PCRs using environmental DNA from freshwater samples."

David N. | Bioinfo Expert

Details

Contents
  • 2X concentrated PCR SuperMix containing reaction buffer with optimized concentrations of molecular-grade MgCl2, dNTPs, AccuStart II hot start Taq DNA polymerase, ToughMix additives and stabilizers.
  • GelTrack Loading Dye: 50X concentrated mixture blue and yellow electrophoresis-tracking dyes. (Does not contain a density reagent.)

Performance Data

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Customer Testimonials

AccuStart II PCR ToughMix

"Good product, worked well on difficult to amplify template."

Research Associate | University of Texas at Austin
AccuStart II PCR ToughMix

"This is an extremely good enzyme mix while testing side by side with Invitrogen's similar enzyme mix. The extension time is ridiculously good with as much as 5 sec for 0.5 kb."

Ph.D. Student | Goethe-Universität Frankfurt am Main
AccuStart II PCR ToughMix

"We had a great result with AccuStart II PCR ToughMix running HRM PCR with tomato crude DNA extract."

Research Scholar | North Carolina State University
AccuStart II PCR ToughMix

"My experiments demonstrated that AccuStart II PCR Toughmix improved the yield of PCRs using environmental DNA from freshwater samples."

David N. | Bioinfo Expert

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